#Clc genomics workbench vs geneious windows

altitudinis 19RS3 genome was assembled into 15 contigs with an N50 value of 1,943,801 bp. The best assembly for both genomes was obtained using Velvet. For genome annotation PROKKA, RAST, and KAAS tools were used. The parameters evaluated were the number of contigs в‰Ґ 500 bp and в‰Ґ 1000 bp, the length of the longest contig, and the N50 value. Assembly evaluation was done by the QUAST tool. Genomes were assembled using SPAdes, ABySS, Velvet, and SOAPdenovo2 assemblers for Linux, and Geneious and CLC Genomics Workbench assemblers for Windows. Raw reads were filtered using the Trimmomatic tool. Library construction and draft genome sequencing were performed by Macrogen services. Both genomes were also automatically annotated to detect PGP genes and compare sequences with other genomes reported.

#Clc genomics workbench vs geneious windows

altitudinis 19RS3 and T5S-T4, using different assemblers available for Windows and Linux and to select the best assembly for each strain. The purposes of this work were to assemble the genomes of B. seedlings and selected for their plant growth-promoting (PGP) properties shown in vitro and in vivo. In our previous studies, two endophytic endospore-forming bacteria identified as Bacillus altitudinis were isolated from roots of Ilex paraguariensis St. PGPB-based inoculant formulation has been used to replace chemical fertilizers and pesticides. Plant growth-promoting bacteria (PGPB) are a heterogeneous group of bacteria that can exert beneficial effects on plant growth directly or indirectly by different mechanisms.